Summary
Type: SPE
Application #: 301700
Catalog#: 730014 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: A)
Column conditioning:
2 column volumes ethyl acetate, then 1 column volume methanol, finally 1 column
volume dist. water
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
1 column volume dist. water; dry column 15 min thoroughly under vacuum
Elution:
2 x 500 μl ethyl acetate; concentrate eluate to about 250 μl in a stream of
nitrogen at 40 °C; if necessary, perform a second purification:
B)
Column conditioning:
200 μl water, then 10 ml n-hexane
Sample application:
Mix concentrated C18 ec eluate with 1 ml n-hexane and force or aspirate it
through the column; collect the eluate
Elution:
10 ml n-hexane; combine eluates: 1st fraction.
Elute a 2nd fraction with benzene / hexane (6 : 4, v/v).
Analyse both fractions separately.
Authors: Macherey-Nagel
Source: LC Department